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Isolation, idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii Mukerjee / 药学学报
Acta Pharmaceutica Sinica ; (12): 780-789, 2013.
Artículo en Chino | WPRIM | ID: wpr-259550
ABSTRACT
A total of 52 endophytic fungi were isolated from roots and stems of Tibetan medicinal plant Phlomis younghusbandii Mukerjee. These fungal isolates were molecularly identified based on ITS sequnces and 28S sequences distributed to 12 genera, including Phoma, Chaetosphaeronema, Fusarium and Leptosphaeria, etc. Among them, the dominant genus was Phoma. Extracts of all strains were evaluated for anti-HIV-1 integrase activity by using soluable integrase expressed in E. coli BL21 (DE3). The results showed that seven samples from five fungal endophytes PHY-24, PHY-38, PHY-40, PHY-51, PHY-53, which belonged to genus Chaetosphaeronema, inhibited strand transfer reaction catalyzed by HIV-1 integrase with IC50 values, of 6.60, 5.20, 2.86, 7.86, 4.47, 4.56 and 3.23 microg x mL(-1) respectively. In conclusion, the endophytic fungi of Phlomis younghusbandii Mukerjee are valuable for further screening anti-HIV-1 integrase agents.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Filogenia / Plantas Medicinales / Plásmidos / Ascomicetos / Proteínas Recombinantes / Chaetomium / Tallos de la Planta / Raíces de Plantas / Inhibidores de Integrasa VIH Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Filogenia / Plantas Medicinales / Plásmidos / Ascomicetos / Proteínas Recombinantes / Chaetomium / Tallos de la Planta / Raíces de Plantas / Inhibidores de Integrasa VIH Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2013 Tipo del documento: Artículo