Construction and eukaryotic expression of PVAX1-hPV58mE6E7fcGB composite gene vaccine / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1102-1107, 2013.
Article
en Zh
| WPRIM
| ID: wpr-259759
Biblioteca responsable:
WPRO
ABSTRACT
To construct and express a composite gene vaccine for human papillomavirus 58(HPV58)-associated cervical cancer, we inserted HPV58mE6E7 fusion gene into pCI-Fc-GPI eukaryotic expression vector, constructing a recombinant plasmid named pCI-sig-HPV58mE6E7-Fc-GPI. Then we further inserted fragment of sig-HPV58mE6E7Fc-GPI into the novel vaccine vector PVAX1-IRES-GM/B7, constructing PVAX1-HPV58mE6E7FcGB composite gene vaccine. PVAX1-HPV58mE6E7FcGB vaccine was successfully constructed and identified by restriction endonuclease and sequencing analysis. Eukaryotic expression of fusion antigen sig-HPV58mE6E7-Fc-GPI and molecular ad-juvant GM-CSF and B7. 1 were proved to be realized at the same time by flow cytometry and immunofluorescence. So PVAX1-HPV58mE6E7FcGB can be taken as a candidate of therapeutic vaccine for HPV58-associated tumors and their precancerous transformations.
Texto completo:
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Índice:
WPRIM
Asunto principal:
Proteínas Recombinantes
/
Neoplasias del Cuello Uterino
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Proteínas Oncogénicas Virales
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Vacunas contra el Cáncer
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Vacunas de ADN
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Proteínas de la Cápside
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Alergia e Inmunología
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Proteínas E7 de Papillomavirus
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Vacunas contra Papillomavirus
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Genética
Límite:
Female
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Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2013
Tipo del documento:
Article