Interaction effect of p53 with HBV in hepatoma cell line SMMC-7721 / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 403-405, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-259970
ABSTRACT
<p><b>OBJECTIVES</b>To study the interaction of hepatitis virus B (HBV) and tumor suppressor p53.</p><p><b>METHODS</b>Plasmid pCMVp53 was transfected or cotransfected with pCMVHBVa (wild type HBV) or PCMVHBVb (mutant type HBV) into the hepatoma cell line SMMC-7721 by lipofectamine. Apoptosis cells were labeled by annexin V-FITC and confirmed by flow cytometry. Reporter plasmids PG13-CAT or p21-luc were cotransfected respectively in each group to indicate transactivation activity of p53 and it's effect on p21 promoter. Western blot was performed to observe p53 expression in each group.</p><p><b>RESULTS</b>The group transfected by pCMVp53 alone exhibit higher luciferase activity and higher apoptosis rate, otherwise, p53 expression, enzyme activity of PG13-CAT or p21- luc and cell apoptosis rate were much higher in the group cotransfected by pCMVp53 and pCMVHBVa, but not in the other cotransfected group; HBV replication was enhanced in p53 cotransfected group.</p><p><b>CONCLUSION</b>p53 expression and effects could be enhanced by HBV and p53 had positive regulation effect on HBV replication.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Virología
/
Replicación Viral
/
Transfección
/
Transactivadores
/
Virus de la Hepatitis B
/
Proteína p53 Supresora de Tumor
/
Proteínas Proto-Oncogénicas p21(ras)
/
Regiones Promotoras Genéticas
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2004
Tipo del documento:
Artículo
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