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Anti-tumor effects of 10-hydroxycamptothecinc-treated DC-Hepa1-6 fusion vaccines / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 344-346, 2004.
Artículo en Chino | WPRIM | ID: wpr-259998
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the induction of antitumor immune responses and therapeutic effects of 10-hydroxycamptothecinc-treated (HCPT) DC-Hepa fusion vaccines by DC fused with hepal-6 cell from hepatoma.</p><p><b>METHODS</b>The fused cells were isolated by magnetic cell sorting and adherent culture. Cell apoptosis was detected by Rhodamine123/PI double-labeled assay, CTL activity by 4 h (51)Cr releasing assay. Protective and therapeutic effects of the fusion vaccine to the tumor-bearing mice was also observed.</p><p><b>RESULTS</b>The apoptosis rate was 29.7%+/-4.1% when DC-Hepa fusion vaccine was treated with 50 microg/ml HCPT for 24 h. After treatment with the HCPT-DC-Hepa fusion vaccine, the tumor grew obviously slowly, survival period of the mice was prolonged, induced more potent CTL cytotoxicity, and resisted against the rechallenge of Hepal-6 cells.</p><p><b>CONCLUSION</b>Vaccination with HCPT-DC-Hepa fusion vaccine could elicit potent antitumor responses, which will provide a new approach to the DC-mediated therapeutic antitumor immunity.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Trasplante / Células Dendríticas / Camptotecina / Células Tumorales Cultivadas / Linfocitos T Citotóxicos / Fusión Celular / Apoptosis / Vacunas contra el Cáncer Límite: Animales Idioma: Chino Revista: Chinese Journal of Hepatology Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Trasplante / Células Dendríticas / Camptotecina / Células Tumorales Cultivadas / Linfocitos T Citotóxicos / Fusión Celular / Apoptosis / Vacunas contra el Cáncer Límite: Animales Idioma: Chino Revista: Chinese Journal of Hepatology Año: 2004 Tipo del documento: Artículo