Expression of cytokines of interleukin-6 family in gingival fibroblasts by Toll-like receptor-2 / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 523-527, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-260244
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether signaling through Toll-like receptor-2 (TLR-2) can affect the expression of some cytokines in human gingival fibroblasts.</p><p><b>METHODS</b>The gingival fibroblasts were isolated and cultured in vivo, divided into blank control group, lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg) group and Escherichia coli (Ec) group. mRNA expression levels were measured by real-time polymerase chain reaction (PCR). The protein expression levels were detected by the enzyme linked immunosorbent assay (ELISA). The data was statistically analyzed by SPSS16.0 software package.</p><p><b>RESULTS</b>LPS from Pg could stimulate the expression of interleukin (IL)-6 and leukemia inhibitory factor (LIF) mRNA and protein, which reached the peak (5.87 ± 0.83) at 10 h, and the expression level increased with the increase of the Pg concentration. IL-11 or oncostatin-M (OSM) mRNA expression was not affected by LPS. After treated with Pg for 48 h, the protein expression of IL-6 and LIF was up-regulated, (962 ± 57) ng/L and (47 ± 18) ng/L respectively.</p><p><b>CONCLUSIONS</b>Signaling through TLR-2 controls the expression of cytokines of IL-6 family in human gingival fibroblasts.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
ARN Mensajero
/
Transducción de Señal
/
Células Cultivadas
/
Química
/
Lipopolisacáridos
/
Interleucina-6
/
Interleucina-11
/
Porphyromonas gingivalis
/
Biología Celular
Límite:
Adolescente
/
Adulto
/
Niño
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2012
Tipo del documento:
Artículo
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