Tissue culture and plant regeneration of Rhodiola henryi / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the tissue culture and plant regeneration technologies and optimizing propagation system in vitro of Rhodiola henryi.</p><p><b>METHOD</b>Orthogonal experiment designs were used in the study of Rh. henryi callus induction, shoot formation and rooting, and the data were analyzed by range analysis and variance analysis.</p><p><b>RESULT</b>The optimal media to induce multiple callus from leaves were MS supplemented with 2,4-D 1.5 mg x L(-1) and 6-BA 0.5 mg x L the effect of the three factors was in sequence of explants > 2,4-D > 6-BA; The optimal media to induce multiple buds from stems were MS supplemented with 6-BA 1.5 mg x L\/1-1 NAA >6-BA; Plantlets were rooted on 1/2MS supplemented with IBA 1.0 mg x L-1, and rooting rate reached to 90% or more and transplant survival rate of plantlet reached 98% or more.</p><p><b>CONCLUSION</b>An efficient system for tissue culture and plant regeneration of Rh. henryi was initially established.</p>