Characterizing effects of solvent specific morus alba components on rat platelet aggregation, vascular tension and macrophage nitrite production / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of different solvent extractions of Mori Ramulus on platelet aggregation, vascular tension, and nitrite production from macrophages stimulated by lipopolysaccharides and interferon-gamma.</p><p><b>METHOD</b>The components of Mori Ramulus were extracted by EtoAc, n-BuOH and chloroform respectively. Platelet aggregation was induced by ADP, arachidonic acid and collagen in vitro; nitrite production of activated macrophages was measured by Griess assay, and the vasodilatory effects of three extractions were investigated by isometric tension changes of aortic rings.</p><p><b>RESULT</b>Chloroform extraction concentration-dependently inhibited platelet aggregation by arachidonic acid, and reduced vascular tension of PE preconstricted aorta rings with or without endothelium. On the other hand, extractions of EtoAc and n-BuOH demonstrated dose-dependent inhibition on macrophage NO production stimulated by LPS/IFN-gamma.</p><p><b>CONCLUSION</b>Pharmacological activities of Mori Ramulus depend on solvent specific components. Chloroform extraction of Mori Ramulus may benefit cardiovascular diseases through its properties of anti-platelet aggregation and vasodilatation. The inhibition of macrophage activity by EtoAc and n-BuOH extractions suggested an anti-inflammation effect of the compound.</p>