Protection and mechanism of total flavone of Hippophae rhamnoides on vascular endothelial cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 355-358, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-260989
ABSTRACT
<p><b>OBJECTIVE</b>To observe the protection of total flavones of Hippophae Rhamnoides (TFH) on vascular endothelial cells (VECs).</p><p><b>METHODS</b>Human umbilical VECs (ECV304) were used. The vascular endothelial injured cell model was prepared using hydrogen dioxide (H2O2). The cell apoptosis rate and changes of mean fluorescence intensity were detected using flow cytometry (FCM). The Caspase-3 activity in VECs was detected by Western blot.</p><p><b>RESULTS</b>VEC apoptosis was induced by 200 micromol/L H2O2. TFH in different concentrations (400, 200, and 100 microg/mL) could significantly lower the cell apoptosis rate induced by H2O2 respectively (all P < 0.05), and obviously inhibit Caspase-3 activities (all P < 0.01).</p><p><b>CONCLUSIONS</b>TFH could fight against H2O2 injured VECs apoptosis. Lowering the Caspase-3 expression was one of its mechanisms in protecting VECs.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Supervivencia Celular
/
Células Cultivadas
/
Apoptosis
/
Sustancias Protectoras
/
Hippophae
/
Células Endoteliales
/
Flavonas
/
Caspasa 3
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2011
Tipo del documento:
Artículo
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