Effect of topically applied recombinant adenovirus of adiponectin on bone defect repair / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 361-365, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-261071
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of local administration of recombinant adenovirus of human adiponectin (hAPN) Ad-hAPN-EGFP on tibial defect repair of SD rats.</p><p><b>METHODS</b>Tibial defect (2 mm) models of 36 SD rats (72 sides) were randomly divided into three groups (A, B, and C; n=24). The three groups were injected with Ad-hAPN-EGFP, Ad-EGFP, and normal saline, respectively, during operation and the day after operation. A week after the operation, the expression of hAPN and osteogenesis-related factors were detected by real-time polymerase chain reaction. Three weeks after operation, the tibias were examined by micro-computed tomography, hematoxylin-eosin staining, and Masson staining to evaluate the restoration of bone defects.</p><p><b>RESULTS</b>1) The hAPN expression was detected in group A but not in groups B and C. Osteogenesis-related factors expression of group A was significantly higher than that of the other groups (P<0.05). 2) Osteogenesis (including bone mineral density, relative bone volume, trabecular number and trabecular thickness) in group A were more evident than those in groups B and C (P<0.05). No significant differences were found between groups B and C (P>0.05).</p><p><b>CONCLUSION</b>Local administration of recombinant adenovirus Ad-hAPN-EGFP may be an effective strategy to improve the restoration of bone defects in vivo.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteogénesis
/
Huesos
/
Densidad Ósea
/
Adenoviridae
/
Ratas Sprague-Dawley
/
Adiponectina
/
Microtomografía por Rayos X
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2015
Tipo del documento:
Artículo
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