Genotypes of adenoviruses in infants and young children with diarrhea / 中国当代儿科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the prevalence of adenoviruses (AdV) and their genotypes in infants and young children with diarrhea.</p><p><b>METHODS</b>A total of 380 children with diarrhea aged less than 3 years were enrolled. The genomic DNA was extracted from stool and PCR was used to detect AdV. Clone sequencing and genotyping were performed for DNA in AdV-positive specimens.</p><p><b>RESULTS</b>AdV was detected in 24 out of 380 specimens, and the detection rate was 6.3% (24/380). A majority of children with positive AdV were aged 2-3 years. The viral sequence analysis of positive specimens showed that the detection rates of enteric AdV41 and non-enteric AdV were 4.2% (16/380) and 2.1% (8/380), respectively, and among the children with non-enteric AdV, there were 2 with AdV1, 2 with AdV2, 1 with AdV7, 2 with AdV12, and 1 with AdV31.</p><p><b>CONCLUSIONS</b>Diarrhea caused by AdV is commonly seen in children aged 2-3 years, and AdV41 is the major predominant strain.</p>