The mechanism of STI571 inducing apoptosis of K562 cells / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the mechanism of STI571 inducing apoptosis of K562 cells which express P210(BCR/ABL).</p><p><b>METHODS</b>Apoptosis was analyzed by Annexin-V/PI, DioC6 [3] staining, DCFH-DA staining, DNA-PI staining and DNA ladder. Western blot was used to analyse mitochondrial and cytosolic cyto C, Bcl-X(L), caspase-3, actin protein and the level of tyrosine phosphorylation.</p><p><b>RESULTS</b>After exposure to STI571, K562 cells were induced to apoptosis. Tyrosine phosphorylation level of P210(BCR/ABL) and Bcl-X(L) was decreased. Caspase-3 was activated and there was an cytosolic accumulation of cyto C.</p><p><b>CONCLUSION</b>STI571 could rapidly decrease the tyrosine phosphorylation level of P210(BCR/ABL). The signal pathway mediated by the cytosolic translocation of mitochondrial cyto C was one of the mechanisms that STI571 inducing apoptosis. STI571 was an effective gene targeting therapeutic agent.</p>