Tumor antigen peptides from HL-60 cells induce specific immune response / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To prepare tumor antigen peptides from HL-60 cells and to induce specific immune response.</p><p><b>METHODS</b>HL-60 antigen peptides were obtained using techniques including freezing and thawing, heat precipitation and acid precipitation. The stimulating effect of the in vitro Hsp70 binding HL-60 peptides on PBMC and the proliferation of stimulated PBMC were observed by T cell activation test. The cytotoxicity of proliferated PBMC is detected by incubating HL-60 cells or K562 cells with PBMC respectively.</p><p><b>RESULTS</b>The obtained tumor antigen peptides were a peptides mixture. The mixed peptides could activate PBMC and cause PBMC proliferation in vitro after presented by Hsp70. The proliferated PBMC showed specific cytotoxicity to HL-60 cells but not to K562 cells.</p><p><b>CONCLUSION</b>The method for preparing of human leukemia tumor antigen peptides used in this paper is simple and easy; the obtained antigen peptides can induce specific immune response in vitro.</p>