Study of in vitro expansion and differentiation into neuron-like cells of human umbilical cord blood mesenchymal stem cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 415-419, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-261421
ABSTRACT
<p><b>OBJECTIVE</b>To explore the isolation, purification and expansion of human umbilical cord blood mesenchymal stem cells (MSCs) into neuron-like cells in vitro.</p><p><b>METHODS</b>Human cord blood samples were obtained sterilely with 20 U/ml preservative-free heparin. MSCs were isolated by lymphocyte separation medium (density 1.077 g/ml), and purified and expanded with Mesencult trade mark medium. The surface antigen expression of MSCs was detected by flow cytometry. The passage 2, 5 and 8 of the expanded MSCs were induced to differentiate to neuron-like cells. Specific markers and structures were detected by immunohistochemistry and histochemistry methods.</p><p><b>RESULTS</b>The number of MSCs increased two- to three-fold with each expanded passage. 6.6 x 10(5) primary MSCs were expanded ten passages to reach a number of 9.9 x 10(8), and was increased about 1.5 x 10(3)-fold. Flow cytometry showed that MSCs did not express antigens CD(34), CD(11a) and CD(11b), but expressed strongly CD(29) and weakly CD(71), which was identical to human bone marrow-derived MSCs. 70% cells exhibited typical neuron-like phenotype after induction. Immunohistochemistry staining showed that all of the induced different-passage MSCs expressed neurofilament (NF) and neuron-specific enolase (NSE). Special Nissl body was found by histochemistry.</p><p><b>CONCLUSION</b>MSCs in human umbilical cord blood can expand in vitro and differentiate into non-mesenchymal cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfopiruvato Hidratasa
/
Células Madre
/
Receptores de Transferrina
/
Inmunohistoquímica
/
Antígenos de Diferenciación de Linfocitos B
/
Antígenos CD
/
Recuento de Células
/
Diferenciación Celular
/
División Celular
/
Química
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Hematology
Año:
2002
Tipo del documento:
Artículo
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