Investigation on quality standard of Franchet groundcherry / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2103-2105, 2010.
Article
en Zh
| WPRIM
| ID: wpr-262215
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop identification and assay methods of Franchet groundcherry.</p><p><b>METHOD</b>TLC method was used to identify of physalin L in the sample using high performance silica gel G plate and a mixture of chloroform-acetone-methanol (25:1:1) as a developing solvent. In the chromatogram, physalin L showed a distinct fluorescence spot under UV 365 nm with good separation. In the HPLC method, luteoloside was separated on a Venusil XBP C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-0. 2% phosphoric acid (20:80) as the mobile phase with flow rate of 1 mL x min(-1). The detection wavelength was set at 350 nm.</p><p><b>RESULT</b>For the HPLC quantitation method, the calibration curve of luteoloside displayed ideal linearity over the range of 0.50-249.40 mg x L(-1) with the regression equation of Y = 55,313X + 3.1641 (r = 1.000). The average recovery of luteoloside was 98.79% with a RSD of 1.1%. And the intra-day and inter-day precisions were less than 2%.</p><p><b>CONCLUSION</b>The TLC identification and HPLC determination were sensitive, reliable and repeatable and can be applied for the quality evaluation and assessment of Franchet Groundcherry Calyx.</p>
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1
Índice:
WPRIM
Asunto principal:
Oligosacáridos
/
Medicamentos Herbarios Chinos
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Cromatografía en Capa Delgada
/
Bignoniaceae
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2010
Tipo del documento:
Article