Modulation of activities and mRNA expression of cytochrome P450 isoenzymes in rat liver by Sophora flaveacens and coadministration with Veratrum nigrum / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1845-1849, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-262243
ABSTRACT
<p><b>OBJECTIVE</b>To study the modulatory effect of Sophora flaveacens and co-administration with Veratrum nigrum on the activity and mRNA expression of cytochrome P450 isoenzymes in rat liver.</p><p><b>METHOD</b>Rat liver microsomal cytochrome P450, b5, aminopyrine N-demethylase (APND), p-nitrophenol-hydroxylase (pNPH) activities were quantitated by UV chromatography. The mRNA expression level of five CYP isoenzymes CYP1A1, CYP2B1/2, CYP2C11, CYP2E1 and CYP3A1 were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULT</b>S. flaveacen and its combination with V. nigrum can dramatically reduce P450 and b5 protein content. Both single and combined use inhibited the activities of aminopyrine N-demethylase. At the mRNA level, the expression of CYP2C11 markedly induced exposure to V. nigrum, while decreased after combination with S. flaveacens. S. flaveacens can induce CYP2B1/2 gene expression, while the Sophora- Veratrum group showed weak inhibition. Both single and co-administrated group have some inhibitory effect on CYP3A1 gene expression, while CYP1A gene expression almost has no change in each group.</p><p><b>CONCLUSION</b>These results indicated that compatibility of Sophora and Veratrum in a prescription also have herb-herb interactions based on P450. The possible mechanisms of its incompatible effects need to be further analysised via integrating with metabolic studies.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Veratrum
/
ARN Mensajero
/
Extractos Vegetales
/
Distribución Aleatoria
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Regulación Enzimológica de la Expresión Génica
/
Química
/
Ratas Wistar
/
Sophora
/
Sistema Enzimático del Citocromo P-450
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2010
Tipo del documento:
Artículo
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