SLC26A4 mutations in a Chinese deafness family discovered by next-generation sequencing technology / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 942-945, 2012.
Article
en Zh
| WPRIM
| ID: wpr-262440
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the molecular etiopathogenesis for a non-syndromic hearing loss patient.</p><p><b>METHODS</b>The core family, consists of the patient and his parents, was recruited. Genomic DNA was extracted from peripheral blood. Mutation analysis was carried out by SNaPshot and next-generation sequencing technology. Mutations in SLC26A4 gene were verified by polymerase chain reaction and direct sequencing.</p><p><b>RESULTS</b>Compound heterozygous mutations p.V306GfsX24 and p.P516PfsX11 in SLC26A4 gene were detected in the patient, heterozygous mutation p.V306GfsX24 was detected in the father, heterozygous mutation p.P516PfsX11 was detected in the mother.</p><p><b>CONCLUSIONS</b>Compound heterozygous mutations p.V306GfsX24 and p.P516PfsX11 contributed to patient's hearing loss. Next-generation sequencing technology is a useful tool for detecting de novo mutations of deafness genes, and is suitable for clinical application.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Linaje
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Proteínas de Transporte de Membrana
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Análisis Mutacional de ADN
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Secuencia de Bases
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Pruebas Genéticas
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Sordera
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Pueblo Asiatico
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Genética
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Tamización de Portadores Genéticos
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Mutación
Límite:
Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Año:
2012
Tipo del documento:
Article