Research of extracting and purifying taxol from the branches of Taxus media / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>The study was to explore extracting and purifying technology of taxol from the branches of Taxus media.</p><p><b>METHOD</b>In extracting phase, the solvent extraction, ultrasonic extraction and the supercritical CO2 fluid extraction were studied respectively; In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane. The content of taxol was detected by HPLC.</p><p><b>RESULT</b>The results showed that the recovery of extracting toxal with the method of ultrasonic was the highest and the selectivity of supercritical CO2 fluid extraction was the best; The sample after being extracted should be purified two stages by silica gel column chromatography with dichloromethane-chloroform-methane (53443) as elution and the lamella chromatography was chloroform-ethyl acetate-methane (8875) as elution, finally we reached the fawn crystal.</p><p><b>CONCLUSION</b>In that production, the content of taxol reached 87. 3% and the recovery 89.7%, which indicat that the taxol is well enrichment.</p>