Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 13-19, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-263230
ABSTRACT
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas Bacterianas
/
Proteínas Recombinantes de Fusión
/
Ingeniería de Proteínas
/
Química
/
Proteína Oncogénica pp60(v-src)
/
Genética
/
Glutatión Transferasa
/
Metabolismo
/
Métodos
Idioma:
Inglés
Revista:
Journal of Zhejiang University. Science. B
Año:
2006
Tipo del documento:
Artículo
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