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Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B ; (12): 13-19, 2006.
Artículo en Inglés | WPRIM | ID: wpr-263230
ABSTRACT
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Saccharomyces cerevisiae / Proteínas Bacterianas / Proteínas Recombinantes de Fusión / Ingeniería de Proteínas / Química / Proteína Oncogénica pp60(v-src) / Genética / Glutatión Transferasa / Metabolismo / Métodos Idioma: Inglés Revista: Journal of Zhejiang University. Science. B Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Saccharomyces cerevisiae / Proteínas Bacterianas / Proteínas Recombinantes de Fusión / Ingeniería de Proteínas / Química / Proteína Oncogénica pp60(v-src) / Genética / Glutatión Transferasa / Metabolismo / Métodos Idioma: Inglés Revista: Journal of Zhejiang University. Science. B Año: 2006 Tipo del documento: Artículo