Bioassay of the soluble human tumor necrosis factor receptor I recombinant plasmid expression in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 185-188, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-263420
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of recombinant plasmid PcDNA3.1-sTNFRI in vitro and evaluate the bioactivity of expressed sTNFRI.</p><p><b>METHODS</b>CHO cells were transfected with recombinant plasmid PcDNA3.1-sTNFRI by liposome. sTNFRI in cell culture supernatant was detected by ELISA and sTNFRI blockage of TNF-alpha cytotoxicity in L929 cells evaluated by MTT assay.</p><p><b>RESULTS</b>The expression of sTNFRI in transfected cell culture supernatant was higher than control groups (P < 0.001). The expressed sTNFRI could significantly neutralize TNF-alpha cytotoxicity in L929 cells.</p><p><b>CONCLUSIONS</b>These results showed that the recombinant plasmid PcDNA3.1-sTNFRI can be expressed in mammalian cells and the recombinant sTNFRI has biological function.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Plásmidos
/
Proteínas Recombinantes de Fusión
/
Transfección
/
Células CHO
/
Clonación Molecular
/
ADN Complementario
/
Receptores Tipo I de Factores de Necrosis Tumoral
/
Células Eucariotas
/
Vectores Genéticos
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2004
Tipo del documento:
Artículo
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