Analysis of differentially expressed lung metastasis-associated proteins in adenoid cystic carcinoma cell lines / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 114-117, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-263443
ABSTRACT
<p><b>OBJECTIVE</b>To analyze differentially expressed metastasis-associated proteins in Adenoid cystic carcinoma cell lines of human salivary gland by proteomics.</p><p><b>METHODS</b>Protein expression alterations of ACC-2 and ACC-M cells were described by 2-D gels. After image analysis by software, proteins of interest were excised from the gels and identified by matrix assisted laser desorption ionization time-of-flight mass spectrometer.</p><p><b>RESULTS</b>12 protein spots showed significantly differential expression patterns between two cell lines. In the identified protein candidates, transketolase, modulator recognition factor 2, Dim1p homolog, splicing factor (arginine/serine-rich 9) and v-Ha-ras l oncogene were all lowly expressed in the poorly metastatic ACC-2 cell and significantly upregulated in highly metastatic ACC-M cell, while type I collagen pro alpha and tumor necrosis factor (ligand) superfamily member 4 showed a high expression in ACC-2 cells and a low expression in ACC-M cells. Pirin (spot 6) just appears in ACC-2 cell and was not detectable in ACC-M cell, while retinal homeobox protein was just detected in ACC-M cell and did not appear in ACC-2 cell.</p><p><b>CONCLUSIONS</b>The proteins may be involved in the adenoid cystic carcinoma lung metastasis through different mechanisms. Our work may contribute to discover diagnostic markers and therapeutic targets.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Nucleares
/
Proteínas Portadoras
/
Carcinoma Adenoide Quístico
/
Proteínas ras
/
Proteómica
/
Línea Celular Tumoral
/
Neoplasias Pulmonares
/
Proteínas de Neoplasias
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2004
Tipo del documento:
Artículo
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