Preparation of genistein-loaded chitosan microspheres / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To optimize the preparation of genistein chitosan microspheres with central composite design (CCD).</p><p><b>METHOD</b>The chitosan microspheres were prepared by the O/W/O multiple emulsion method. Second-order polynomial and linear equations were fitted to the data, and the resulting equations were used to produce response surface graphs and the best experiment conditions.</p><p><b>RESULT</b>The theoretical drug content was 13%-15%, the concentration of organic phase was 30%-40% and the concentration of oil phase was 68%-72%.</p><p><b>CONCLUSION</b>The best experiment conditions can be obtained by central composite design and response surface methodology. The observed values agree well with model predicted values.</p>