Study on stem-tip tissue culture of the traditional Chinese medicine Chrysanthemum morifolium / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To set up the optimums for the stem-tip tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.</p><p><b>METHOD</b>Small sections (about 0.5 mm in length) from the stem-tips were isolated and inoculated with different media, and induced to form the whole plantlet formation.</p><p><b>RESULT AND CONCLUSION</b>The MS medium added with 6-BA 2 mg.L-1 + NAA 0.2 mg.L-1 was the optimum medium for the bud sprouting and the inducing rate was over 80% after 40 d cultivation on this modified medium. The MS medium supplemented with 6-BA 2 mg.L-1 and NAA 0.5 mg.L-1 was the optimum medium for the multiplication of the adventitious buds in which the bud multiplication was about 4-7 times higher after 25-30 d cultivation. The plantlet could root well on the MS medium with NAA 0.5 mg.L-1.</p>