Sequence analysis of a novel HLA-DRB1 allele, DRB1 * 1212 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 47-49, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-263855
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular genetics basis for HLA novel allele HLA-DRB1*1212 in Chinese population.</p><p><b>METHODS</b>Genomic DNA was extracted from whole blood by salting-out method. HLA-DRB1 gene exon 2 was amplified by PCR with group-specific primers from genomic DNA. PCR products were cut back from agarose gels and purified to sequence directly. The polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO) was performed to confirm the mutations which were detected by sequencing in this study.</p><p><b>RESULTS</b>The sequencing results showed HLA-DRB1 alleles of the proband as DRB1*090102 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY899825). Through BLAST analysis, the novel allele was found to be different from DRB1*120101 at position 199A-->C in exon 2, that results in an amino acid change from Ile to Leu at codon 67.</p><p><b>CONCLUSION</b>This allele is a novel and has been officially named as DRB1*1212 by the WHO Nomenclature Committee.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Etnicidad
/
Antígenos HLA-DR
/
China
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia
/
Pueblo Asiatico
/
Alelos
Límite:
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2006
Tipo del documento:
Artículo
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