Establishment of two-dimensional electrophoresis system of caudal gland / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2542-2547, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-263890
ABSTRACT
<p><b>OBJECTIVE</b>To establish an effective separation system of 2-DE for the proteome of caudal gland, and provide foundation for revealing the mechanisms of histological development and pharmacological activities.</p><p><b>METHOD</b>The total proteins of caudal gland were extracted by TCA/acetone precipitation, phenol extraction/methanol-ammonium acetate precipitation and trizol-base method respectively and separated by immobilized pH gradient (IPG) strips prior to SDS-PAGE. Loading protein sample size and isoelectric focusing conditions were optimized. The gels were stained with Coomassie brilliant blue, scanned and then analyzed using PDQuest 8.0 analysis software.</p><p><b>RESULT</b>The total proteins of caudal gland extracted by trizol-base method were the highest quality and could meet the needs of 2-DE. With 300 microg of proteins loaded on 7 cm pH 3-10 IPG strip followed by isoelectric focusing program II ,a satisfying 2-DE profiles were obtained. The total number of disticted protein spots was 209 with the optimized system.</p><p><b>CONCLUSION</b>A well-resolved 2-DE patterns of caudal gland were obtained by this optimized system. This method could be applied to prepare other similar tissue sample and 2-DE studies.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Glándulas Odoríferas
/
Ciervos
/
Electroforesis en Gel Bidimensional
/
Proteínas
/
Química
/
Métodos
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Artículo
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