MiR-135b promotes proliferation of endometrial carcinoma cells by targeting FOXO1 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 675-680, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-263982
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of miR-135b in endometrial carcinoma and the mechanism by which miR-135b promotes the proliferation of endometrial cancer cells.</p><p><b>METHODS</b>The expressions of miR-135b and FOXO1 were using RT-PCR detected in 22 fresh endometrial cancer tissues and paired adjacent tissues and also in endometrial cancer cell lines JEC, Ishikawa, HEC-1-B, and RL-952. The RL-952 and Ishikawa cell lines were transfected with miR-135b mimics or inhibitors, and the changes in their proliferative activity were detected with MTT assay; the expressions of FOXO1 mRNA and protein were detected by RT-PCR and Western blotting, respectively.</p><p><b>RESULTS</b>The expression of miRNA135b was significantly up-regulated and FOXO1 expression was down-regulated in endometrial carcinoma tissues as compared with the adjacent tissues (P<0.05). The mRNA expression of miR-135b was negatively correlated with the expression of FOXO1 in endometrial carcinoma. In RL-952 and Ishikawa cell lines, transfection with miR-135b mimics obviously promoted the cell proliferation (P<0.05). Up-regulation of miR-135b significantly decreased the expressions of FOXO1 protein and mRNA (P<0.05), and down- regulation of miR-135b increased FOXO1 expressions (P<0.05).</p><p><b>CONCLUSIONS</b>MiR-135b plays an important role in the occurrence and development of endometrial carcinoma partially by regulating its target gene FOXO1.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Transfección
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Regulación hacia Abajo
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Regulación Neoplásica de la Expresión Génica
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Regulación hacia Arriba
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Neoplasias Endometriales
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MicroARNs
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Línea Celular Tumoral
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Proliferación Celular
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Factores de Transcripción Forkhead
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2016
Tipo del documento:
Artículo
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