Effects of exendin-4 on extracellular matrix metabolism in human mesangial cells cultured in high glucose / 南方医科大学学报
Journal of Southern Medical University
;
(12): 371-374, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-264038
ABSTRACT
<p><b>OBJECTIVE</b>To explore effects of exendin-4 on the metabolism of extracellular matrix (ECM) in human mesangial cells (HMC) cultured in the presence of high glucose and explore the possible mechanism.</p><p><b>METHODS</b>Human mesangial cells (HMC) were treated with exendin-4 under high glucose conditions. The cell proliferation was observed using CCK8 assay, and the expressions of collagen type I, fibronectin, transforming growth factor-β1 (TGFβ1) expression and extracellular signal- regulated kinase (ERK) signaling pathway activity were assessed using Western blotting.</p><p><b>RESULTS</b>Exendin-4 inhibited cell proliferation and the expressions of collagen type I, fibronectin and TGFβ1 and reversed ERK phosphorylation in high glucose-induced HMC.</p><p><b>CONCLUSION</b>Exendin-4 can regulate ECM metabolism in HMC cultured in high glucose by inhibiting TGFβ1/ERK pathway, suggesting the beneficial effects of exendin-4 in preventing and treating diabetic nephropathy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Péptidos
/
Farmacología
/
Fosforilación
/
Ponzoñas
/
Transducción de Señal
/
Células Cultivadas
/
Química
/
Fibronectinas
/
Sistema de Señalización de MAP Quinasas
/
Medios de Cultivo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2016
Tipo del documento:
Artículo
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