Real-time RT-PCR Assay for the detection of Tahyna Virus / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 374-377, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-264574
ABSTRACT
A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequences encoding the nucleocapsid protein from the Tahyna virus. Primers and probes were selected in conserved regions by aligning genetic sequences from various Tahyna virus strains available from GenBank. The sensitivity of the RT-qPCR approach was compared to that of a standard plaque assay in BHK cells. RT-qPCR assay can detect 4.8 PFU of titrated Tahyna virus. Assay specificities were determined by testing a battery of arboviruses, including representative strains of Tahyna virus and other arthropod-borne viruses from China. Seven strains of Tahyna virus were confirmed as positive; the other seven species of arboviruses could not be detected by RT-qPCR. Additionally, the assay was used to detect Tahyna viral RNA in pooled mosquito samples. The RT-qPCR assay detected Tahyna virus in a sensitive, specific, and rapid manner; these findings support the use of the assay in viral surveillance.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Virus de la Encefalitis de California
/
Sensibilidad y Especificidad
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Métodos
/
Culicidae
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2015
Tipo del documento:
Artículo
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