Visual detection of murray valley encephalitis virus by reverse transcription loop-mediated isothermal amplification / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 227-230, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-264595
ABSTRACT
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 °C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transcripción Genética
/
Secuencia de Bases
/
Virus de la Encefalitis del Valle Murray
/
Cartilla de ADN
/
Límite de Detección
/
Genética
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2015
Tipo del documento:
Artículo
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