Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel / 神经科学通报·英文版

ABSTRACT

<p><b>OBJECTIVE</b>To examine the effect of deglycosylation on gating properties of rNav1.3.</p><p><b>METHODS</b>rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control.</p><p><b>RESULTS</b>Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV.</p><p><b>CONCLUSION</b>Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.</p>