Monosaccharide composition analysis and its content determination of polysaccharides from Rhaponticum uniforum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide, and provide some references for further research.</p><p><b>METHOD</b>The monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Phenol-sulfuric acid method was used for the determination of the content of polysaccharide.</p><p><b>RESULT</b>The monosaccharides composition in polysaccharides from R. uniforum are glucose, arabonose and fructose. Their molar ratios are 1 1.61 2.21. The content of polysaccharide is 95.78%, taking the mixture of monosaccharide compositions as reference substances.</p><p><b>CONCLUSION</b>HPAEC-PAD can be used to analyze the monosaccharide composition in the polysaccharide with high precision, and the method of phenol-sulfuric acid is simple, convenient and reliable.</p>