Optimization of conditions for human erythropoiesis in human-mouse xenotransplant model / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1606-1611, 2013.
Article
en Zh
| WPRIM
| ID: wpr-264967
Biblioteca responsable:
WPRO
ABSTRACT
This study was purposed to investigate the conditions for improving human-mouse xenograft and the erythroid differentiation of human hematopoietic stem cells (HSC) in the xenotransplant model. The engraftments of different mouse strains (NOD/SCID or NOD/SCID/IL2rγ(null)), schemes of irradiation (single-time or 2-times radiation; Co(60)γ-ray or X-ray) and strategies of CB CD34(+) cells ex vivo culture time and lentivirus infection were compared. The results showed that at 4 weeks after transplantation, the ratio of hCD45 positive cells in bone marrow of NOD/SCID/IL2rγ(null) mice increased to (51.4 ± 13.9)%, and erythroid precursor could be detected. All of the mice receiving X-ray irradiation for 2 times (a dose of 1 Gy, then the second of 1.5 Gy, with an interval of 15 min) survived. Fresh isolated CB CD34(+) cells were cultured and infected with lentivirus for 72 h and then transplanted into receptor mouse. After 4 weeks, higher engraftment [hCD45 (51.4 ± 13.9)%] and better erythroid development [hCD71(+) GPA(+) (5.98 ± 3.46)%] were observed. It is concluded that NOD/SCID/IL2rγ(null) mice receiving X-ray irradiation for 2 times and were injected with fresh isolated CB CD34(+) cells cultured and infected with lentivirus ex vivo within 72 h show a better xenograft and erythroid development.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Trasplante Heterólogo
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Células de la Médula Ósea
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Células Cultivadas
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Ratones SCID
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Ratones Endogámicos NOD
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Trasplante de Células Madre Hematopoyéticas
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Antígenos CD34
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Biología Celular
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Eritrocitos
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Eritropoyesis
Límite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2013
Tipo del documento:
Article