Your browser doesn't support javascript.
loading
Effects of power frequency magnetic field on gap junction intercellular communication of astrocytes / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 132-134, 2003.
Artículo en Chino | WPRIM | ID: wpr-265036
ABSTRACT
<p><b>OBJECTIVE</b>In order to explore if power frequency magnetic field (PFMF) can act as cancer promoter or be synergistic with phorbol 12-myristate 13-acetate (TPA) in cancer promotion, the effects of 50 Hz MF on gap junction intercellular communication (GJIC) of astrocytes were observed.</p><p><b>METHODS</b>Fluorescence redistribution after photobleaching (FRAP) was adopted to observe the recovery of fluorescence intensity in the bleached cells thus to estimate intercellular communication by gap junction. Comparative fluorescence intensity recovery rate (CFIRR) was as evaluation index. The effects of 50 Hz MF alone or with TPA on GJIC of astrocytes were studied.</p><p><b>RESULTS</b>After 3 ng/ml TPA treatment for 1 hour, M(d) of CFIRR was 4.53%/min, whereas that in the control group was 9.74%/min (H = 12.084, P < 0.005). After exposure to 0.8 and 1.6 mT magnetic field for 24 hours respectively, M(d) of CFIRR was 8.25%/min and 6.68%/min respectively, no significant difference from that of control (H = 32.617, P > 0.05). After exposure to 0.8 and 1.6 mT magnetic field for 23 hours then combined with 3 ng/ml TPA treatment for 1 hour, M(d) of CFIRR was 3.32%/min and 2.85%/min respectively, also no significant difference from that in the group treated with 3 ng/ml TPA alone (H = 2.589, P > 0.05).</p><p><b>CONCLUSION</b>50 Hz MF (within 0 - 1.6 mT) alone could not inhibit GJIC of astrocytes; with TPA, could not enhance the inhibition of TPA on GJIC of astrocytes. But with MF intensity increasing, the inhibition of MF on GJIC showed elevated tendency.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ornitina Descarboxilasa / Farmacología / Efectos de la Radiación / Acetato de Tetradecanoilforbol / Comunicación Celular / Astrocitos / Ratas Sprague-Dawley / Uniones Comunicantes / Campos Electromagnéticos / Metabolismo Límite: Animales Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2003 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ornitina Descarboxilasa / Farmacología / Efectos de la Radiación / Acetato de Tetradecanoilforbol / Comunicación Celular / Astrocitos / Ratas Sprague-Dawley / Uniones Comunicantes / Campos Electromagnéticos / Metabolismo Límite: Animales Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2003 Tipo del documento: Artículo