Sudy on the activation of early growth response factor-1 by silica dioxide and its signal pathway / 中华病理学杂志
Chinese Journal of Pathology
; (12): 293-296, 2005.
Article
en Zh
| WPRIM
| ID: wpr-265122
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To discuss the role of early growth response factor (Egr)-1 and it's upstream signaling pathway in the development of silicosis.</p><p><b>METHODS</b>The expression and localization of Egr-1 were analyzed by immunofluorescence and in-situ hybridization. The activity of Egr-1 was observed in treated cells by using a reporter plasmid and EMSA, the activity of ERK1/2 in RAW264.7 incubated with SiO(2) by using a kinase assay, and further by using a kinase inhibitor assay to investigate the role of upstream kinase in the signal pathway of the activation of Egr-1.</p><p><b>RESULTS</b>The obvious increase of expression and transcription of Egr-1 was observed shortly after being treated by silica and its activity increased abruptly. There was an increase of the activity of ERK1/2 in RAW264.7 cells treated, which reached a peak at 30 minutes. The expression and transcription of Egr-1 decreased maniferstly after using kinase inhibitors.</p><p><b>CONCLUSION</b>Egr-1 expression can be induced by silica dioxide in RAW264.7 cells, and the ERK1/2, p38 kinases may take part in this process which suggest the pathway of SiO(2), ERK1/2, p38 and Egr-1 may play an important role in the development of silicosis.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
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Fisiología
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Butadienos
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ARN Mensajero
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Transducción de Señal
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Células Cultivadas
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Regulación de la Expresión Génica
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Dióxido de Silicio
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Proteína Quinasa 1 Activada por Mitógenos
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Proteína Quinasa 3 Activada por Mitógenos
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Año:
2005
Tipo del documento:
Article