Malignant transformation of NIH3T3 cells induced by ectopic expression of PC-1 gene / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 42-46, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-265201
ABSTRACT
<p><b>OBJECTIVE</b>To establish a mouse fibroblastic cell line stably transfected with PC-1 gene, and using such cell line to investigate tumor development and progression imposed by the ectopic expression of PC-1 gene.</p><p><b>METHODS</b>Eukaryotic expression vector pcDNA3.1(-)/myc-his-pc-1 was transfected into mouse fibroblast cell line NIH3T3 by lipofectamine. Stable transfectants were selected by G418. The integration and expression of ectopic PC-1 gene were analyzed by PCR and RT-PCR. Cytomorphological analysis, MTT, soft agar colony formation and nude mice tumorigenesis assay were used to evaluate the effects of PC-1 gene expression on tumor development and progression.</p><p><b>RESULTS</b>NIH 3T3 cell lines stably expressing PC-1 gene were successfully established and confirmed by PCR and RT-PCR analyses of the integration and expression of ectopic PC-1 gene. Comparing with the parental cell line and cells transfected with control vector, the PC-1 gene transfectants acquired several phenotypes of transformed cells increasing growth rate, ability to grow and form cell colonies on soft agar, and becoming tumorigenic in nude mice.</p><p><b>CONCLUSION</b>Ectopic expression of PC-1 gene in NIH3T3 cells can induce malignant transformation of mouse fibroblastic cells both in vitro and in vivo.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
ARN Mensajero
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Línea Celular Transformada
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Transfección
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Distribución Aleatoria
/
Expresión Génica
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Transformación Celular Neoplásica
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Células 3T3 NIH
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Genes Relacionados con las Neoplasias
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Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2005
Tipo del documento:
Artículo
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