Detection of FUS-CHOP fusion gene in paraffin-embedded tissues and its clinicopathologic significance for myxoid/round cell liposarcomas / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 28-32, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-265204
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of detecting FUS-CHOP fusion gene in formalin-fixed, paraffin-embedded tissue and its application in the diagnosis and differential diagnosis of myxoid/round cell liposarcomas (MRCLs).</p><p><b>METHODS</b>Forty-four formalin-fixed, paraffin-embedded MRCL samples and 60 control cases (atypical/well-differentiated liposarcoma, pleomorphic liposarcoma, low-grade myofibrosarcoma, etc.) retrieved from the archival files were studied. Nested reverse transcription-polymerase chain reaction (RT-PCR) technique was employed to detect the FUS-CHOP mRNA expression, followed by DNA sequencing confirmation of the PCR product. Housekeeping gene PGK was used to assess the quality of the mRNA templates.</p><p><b>RESULTS</b>PGK mRNA was detected in 93 of 104 tumor cases (89.4%), including 39 MRCLs cases (39/44, 88.6%) and 90% of the negative control cases. Type II FUS-CHOP fusion transcript was successfully detected in 20 out of 39 (51.3%) MRCL cases. Type I FUS-CHOP fusion transcript was not detected in any MRCLs in this study. All 60 negative control cases were negative for the FUS-CHOP fusion gene transcripts.</p><p><b>CONCLUSIONS</b>(1) Nested RT-PCR can be used to detect FUS-CHOP mRNA in formalin-fixed, paraffin-embedded tissues. (2) FUS-CHOP is considered a specific molecular and genetic hallmark for MRCLs. Nested RT-PCR is a sensitive and specific technique in detecting FUS-CHOP gene, and can be used in the diagnosis and differential diagnosis of MRCLs.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias de los Tejidos Blandos
/
ARN Mensajero
/
Biomarcadores de Tumor
/
Proteínas de Fusión Oncogénica
/
Adhesión en Parafina
/
Liposarcoma Mixoide
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Extremidad Inferior
/
Proteína FUS de Unión a ARN
Tipo de estudio:
Estudio diagnóstico
Límite:
Adolescente
/
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2005
Tipo del documento:
Artículo
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