Determination of total saponin in Semen Platycladi and defatted powder of Semen Platycladi / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the contents of total saponin in the unprocessed Semen Platycladi and defatted powder of Semen Platycladi.</p><p><b>METHOD</b>Through contrasting different coloration, proper condition was selected by using orthogonal test design. Spectrophotometry was established to determine the contents of total saponin in the unprocessed Semen Platycladi and defatted powder of Semen Platycladi.</p><p><b>RESULT</b>Coloration system of vanillin-acetic acid-sulfuric acid was determined. The proper condition of coloration was that sulfuric acid was 80%, water bathed 85 and heated for 10 minutes. The contents of total saponin in unprocessed Semen Platycladi was 0.142% the content of total saponin in the defatted powder of Semen Platycladi was 0. 319%.</p><p><b>CONCLUSION</b>This method, stable, simple and quick, is applicable to the determination of total saponin in Semen Platycladi. A Little of total saponin is lost in the processing course of Semen Platycladi.</p>