Characteristics of P2X purinoceptors in the membrane of rat trigeminal ganglion neurons / 生理学报
Acta Physiologica Sinica
;
(6): 164-170, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-265470
ABSTRACT
The characteristics of purinoceptors in the membrane of rat trigeminal ganglion (TG) neurons were studied by using whole- cell patch clamp technique. The results showed that most of neurons examined (78.9%, 142/180) were responsive to ATP in a concentration-dependent manner; the others (21.1%, 38/180) were ATP insensitive. Of the ATP-sensitive cells, the majority (95.1%, 135/142) responded to ATP with an inward current, a few (2.1%, 3/142) with an outward current, and the rest (2.8%, 4/142) with biphasic current. Small sized cells (<30 mum) responded to ATP with a rapid desensitizing inward current and were highly sensitive to vanilloid; the medium sized cells (30~40 mum) responded to ATP with slow desensitizing inward current and were not sensitive to vanilloid; while the majority of large sized cells (>40 mum) did not respond to ATP and vanilloid. The waveform of ATP-activated inward currents was related to the cell diameter. The I-V curves for both small and medium sized cells manifested obvious inward rectification. Furthermore, we studied the kinetic features of ATP-activated currents and the effects of P2 purinoceptor agonists and antagonists on I(ATP). The findings suggest that ATP receptor-ion channels are expressed differently among different types of rat TG neurons.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Adenosina Trifosfato
/
Ganglio del Trigémino
/
Ratas Sprague-Dawley
/
Técnicas de Placa-Clamp
/
Biología Celular
/
Receptores Purinérgicos P2X
/
Animales Recién Nacidos
/
Metabolismo
/
Neuronas
Límite:
Animales
Idioma:
Inglés
Revista:
Acta Physiologica Sinica
Año:
2006
Tipo del documento:
Artículo
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