Your browser doesn't support javascript.
loading
Telomerase: the expression and regulatory mechanisms in preovulatory ovarian granulosa cells / 生理学报
Acta Physiologica Sinica ; (6): 714-718, 2005.
Artículo en Chino | WPRIM | ID: wpr-265505
ABSTRACT
The objective of this study was to analyze the expression of telomerase in granulosa cells and the influential factors in telomerase expression. TRAP-ELISA (telomeric repeat amplification protocol-enzyme linked immunoadsordent assay) method was used to study the expression and control of telomerase in rat preovulatory ovary. We also used radioimmunoassay (RIA) to determine the expression of estradiol (E(2)) and progesterone (P(0)). Furthermore we used MTT to study the proliferation of ovarian granulosa cells. Telomerase activity was significantly enhanced by human chorionic gonadotropin (HCG), follicle-stimulating hormone (FSH), verapamil and dbcAMP, and was significantly reduced by antisense-c-myb oligodeoxynucleotide (anti-c-myb ODN) treatment. RIA was used to determine the secretion of P(0) and E(2) in all these cell culture media. We found that the secretion of these two hormones was increased when verapamil and FSH were added; no change after adding HCG and dbcAMP; and reduced when anti-c-myb was added. In MTT assay, we found that the antisense hTERT ODN significantly inhibited the proliferation of ovarian granulosa cells. These results demonstrate that telomerase activity is present in ovary antral granulosa cells and its activity is controlled by FSH, HCG, verapamil and anti-c-myb, and is directly related with the function of proliferation.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ovario / Farmacología / Fisiología / Verapamilo / Células Cultivadas / Ratas Sprague-Dawley / Telomerasa / Biología Celular / Proliferación Celular / Hormona Folículo Estimulante Tipo de estudio: Guía de Práctica Clínica Límite: Animales / Femenino / Humanos Idioma: Chino Revista: Acta Physiologica Sinica Año: 2005 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ovario / Farmacología / Fisiología / Verapamilo / Células Cultivadas / Ratas Sprague-Dawley / Telomerasa / Biología Celular / Proliferación Celular / Hormona Folículo Estimulante Tipo de estudio: Guía de Práctica Clínica Límite: Animales / Femenino / Humanos Idioma: Chino Revista: Acta Physiologica Sinica Año: 2005 Tipo del documento: Artículo