Effect of retinoic acid on alkaline phosphatase expression in rat adipose-derived stem cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 32-36, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-265702
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether adipose-derived stem cells (ADSCs) induced by retinoic acid (RA) in vitro express primordial germ cell marker alkaline phosphatase (ALP) and vasa.</p><p><b>METHODS</b>ADSCs were isolated from adult female SD rats and cultured in vitro. The third passage of ADSCs was identified by adipogenic differentiation, osteogenic differentiation and cell surface marker detection. The ADSCs were treated with 1×10(-5), 1×10(-6), or 1×10(-7) mol/L RA for 7 or 14 days, and the cellular expression of ALP was detected. vasa mRNA expression in ADSCs treated with 1×10(-5) mol/L RA for 7 days was detected using RT-PCR.</p><p><b>RESULTS</b>The OD value of ADSCs treated with 1×10(-5), 1×10(-6), or 1×10(-7) mol/L RA was 0.59∓0.04, 0.27∓0.07, and 0.15∓0.03 after a 7-day treatment, and was 0.42∓0.02, 0.34∓0.01, and 0.19∓0.02 after a 14-day treatment, respectively, demonstrating significantly enhanced ALP expression in RA-treated ADSCs compared with that in the control cells (0.07∓0.01 and 0.07∓0.01 at 7 and 14 days, respectively, P<0.01). The ADSCs showed a negative vasa mRNA expression after 1×10(-5) mol/L RA treatment for 7 days.</p><p><b>CONCLUSION</b>RA-induced ADSCs express ALP, a marker of primordial germ cells, but does not express the primordial germ cell marker vasa.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Tretinoina
/
Diferenciación Celular
/
Células Cultivadas
/
Tejido Adiposo
/
Ratas Sprague-Dawley
/
Biología Celular
/
Fosfatasa Alcalina
/
Células Madre Adultas
/
Células Germinativas
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Artículo
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