Effect of lentivirus-mediated nestin gene silencing on metastatic potential of human melanoma cell line UACC903 in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 7-13, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-265707
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of lentivirus-mediated RNA interference (RNAi) of nestin on the metastatic potential of human melanoma cell line UACC903.</p><p><b>METHODS</b>A lentiviral vector for RNAi targeting the coding region of human nestin mRNA (nestin-RNAi-LV) and another control vector containing a nonsense sequence were constructed. The vectors were transfected into UACC903 cells, and nestin expression in the cells was detected by RT-PCR, immunofluorence assay and Western blotting. The invasive ability and migration of the transfected UACC903 cells was evaluated using Transwell and scrape assay, respectively. Fluorescence assay was used to examine the expressions of E-cadherin, N-cadherin and β-catenin in the cells.</p><p><b>RESULTS</b>The lentiviral vector nestin-RNAi-LV was constructed successfully. Compared with the control vector, nestin-RNAi-LV resulted in obviously reduced expression of nestin mRNA and protein, lowered migration ability of UACC903 cells, and reduced cell adhesion and invasiveness (P<0.05).</p><p><b>CONCLUSION</b>Lentivirus-mediated nestin RNAi can specifically inhibit nestin expression to cause decreased cell migration and invasiveness of human melanoma cell line UACC903.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
ARN Mensajero
/
Movimiento Celular
/
Lentivirus
/
ARN Interferente Pequeño
/
Interferencia de ARN
/
Línea Celular Tumoral
/
Nestina
/
Vectores Genéticos
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Artículo
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