Antagonistic effect of myricetin on platelet activing factor / 药学学报
Acta Pharmaceutica Sinica
;
(12): 831-833, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-266574
ABSTRACT
<p><b>AIM</b>To study the antagonistic effect of myricetin on platelet activing factor (PAF).</p><p><b>METHODS</b>The specific binding of [3H] PAF to rabbit platelet receptor was investigated using radio ligand binding assay (RLBA). Platelet adhesion induced by PAF was measured with spectrophotometry. The elevation of inner free calcium concentration in rabbit polymorphonuclear leukocytes (PMNs) induced by PAF was assayed by Fura-2 fluorescent technique.</p><p><b>RESULTS</b>The specific binding inhibition potency of Myr was found to be concentration-dependent. The IC50 of Myr in [3H] PAF 1, 2 and 4 nmol.L-1 were 34.8, 85.7 and 118.6 mumol.L-1, respectively. The PAF induced reactions of rabbit platelet adhesion and PMNs inner free calcium concentration increase were inhibited by Myr in a dose-dependent manner. The IC50 of Myr to inhibit platelet adhesion was 13.1 mumol.L-1.</p><p><b>CONCLUSION</b>The specific receptor binding of PAF can be antagonized by myricetin.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Flavonoides
/
Inhibidores de Agregación Plaquetaria
/
Factor de Activación Plaquetaria
/
Glicoproteínas de Membrana Plaquetaria
/
Activación Plaquetaria
/
Adhesividad Plaquetaria
/
Calcio
/
Receptores Acoplados a Proteínas G
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2003
Tipo del documento:
Artículo
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