Analysis on establishment and affecting factors of qi stagnation and blood stasis rat model / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study on the method for establishing the Qi stagnation and blood stasis rat model and analyze the affecting factors.</p><p><b>METHOD</b>The orthogonal design was adopted to study the influences of joint stimulations including noise, light, electricity, ice water bath, tail-clamping on model rats. The 'flying spot' method was used to dynamically simulate blood flow velocity in microcirculation. the pressure sensing technology of MOTO was adopted to detect hemorheology-related indicators. And the coagulation method was used to detect blood coagulation-related indicators.</p><p><b>RESULT</b>Compared with the negative control group, all model groups showed significant reduction in the blood flow velocity in mesenteric microcirculation and increase in the whole blood viscosity at high, medium and low shear rate, the plasma viscosity and the fibrinogen content in four blood coagulation indicators.</p><p><b>CONCLUSION</b>Noise, light, electricity, tail-clamping, bondage and icewater-bath make significant impact on model rats.</p>