Effects of Fengbaisan on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with chronic obstructive pulmonary disease / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 224-231, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-267185
ABSTRACT
<p><b>OBJECTIVE</b>To observe effects of Fengbaisan (, FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of FBS.</p><p><b>METHODS</b>The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry.</p><p><b>RESULTS</b>The typical histopathological changes of COPD were displayed in the model group, Ambroxol Hydrochloride group and FBS group, and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P<0.05). After treatment for successive 28 days, the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P<0.05).</p><p><b>CONCLUSIONS</b>FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats, and this may be a possible therapeutic mechanism of FBS on COPD.</p>
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
ARN Mensajero
/
Medicamentos Herbarios Chinos
/
Inmunohistoquímica
/
Regulación Enzimológica de la Expresión Génica
/
Ratas Wistar
/
Inhibidor Tisular de Metaloproteinasa-1
/
Metaloproteinasa 9 de la Matriz
/
Enfermedad Pulmonar Obstructiva Crónica
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese journal of integrative medicine
Año:
2014
Tipo del documento:
Artículo
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