Oxidation status and γ-glutamylcysteine synthetase expression in the kidney of MRL/lpr lupus mice / 南方医科大学学报
Journal of Southern Medical University
;
(12): 138-140, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-267653
ABSTRACT
<p><b>OBJECTIVE</b>[corrected] To investigate the role of glutathione (GSH) and γ-glutamylcysteine synthetase (γ-GCS) in lupus nephritis.</p><p><b>METHODS</b>Spectrophotometry was used to measure the oxidative/anti-oxidative indices including malonyldialdehyed (MDA) and GSH in the kidney of MRL/lpr lupus mice. Quantitative PCR and Western blotting were used to detected the expression of γ-GCS.</p><p><b>RESULTS</b>The level of GSH was lowered whereas the level of MDA increased significantly in the kidney tissue of MRL/lpr lupus mice as compared with that in normal control mice. The expression of γ-GCS mRNA and protein was significantly decreased in MRL/lpr lupus mice (P<0.05).</p><p><b>CONCLUSION</b>MRL/lpr lupus mice have abnormal oxidative stress in the kidney tissue, where the expression of γ-GCS decreased to lead to reduced GSH production, damaged antioxidative capacity, and eventually exacerbation of oxidative damage in the kidney.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Nefritis Lúpica
/
ARN Mensajero
/
Estrés Oxidativo
/
Ratones Endogámicos MRL lpr
/
Glutamato-Cisteína Ligasa
/
Genética
/
Glutatión
/
Riñón
/
Malondialdehído
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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