Effect of Shuyusan on neuropeptide Y and serotonin expression in the hippocampal neurons of rats with chronic mild unpredictable stress-induced depression / 南方医科大学学报
Journal of Southern Medical University
;
(12): 113-116, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-267659
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Shuyusan decoction on neuropeptide Y (NPY) and serotonin (5-HT) expression in the hippocampus and plasma of rats with chronic mild unpredictable stressors depression.</p><p><b>METHODS</b>Fifty Wistar rats were randomly divided into 5 groups, namely the normal control group, model group, fluoxetine group, and high- and low-dose Shuyusan groups. Except for those in the normal control group, all the rats were subjected to chronic mild unpredicted stress for 21 consecutive days with corresponding treatments. Open-field test was used to assess the behavioral changes of the rats. The content of NPY in the hippocampus and plasma was detected by competitive enzyme-linked immunosorbent assay, and immunocytochemistry was used to determine the expression of 5-HT in the hippocampus.</p><p><b>RESULTS</b>NPY levels in the hippocampus and plasma was significantly decreased in the model group as compared with that in the normal control group (P<0.05). Treatments with fluoxetine and high-dose Shuyusan both significantly increased NPY levels in the hippocampus and plasma in the depressive rats (P<0.05), resulting also in significantly increased 5-HT-immunoreactive neurons in the cerebral cortex and hippocampus and the average optical density (P<0.05).</p><p><b>CONCLUSION</b>Shuyusan decoction can effectively increase plasma and hippocampus NPY levels and the number of 5-HT-positive neurons in the cerebral cortex and happocampus of rats with chronic mild unpredictable stress-induced depression.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Estrés Fisiológico
/
Neuropéptido Y
/
Medicamentos Herbarios Chinos
/
Distribución Aleatoria
/
Serotonina
/
Ratas Wistar
/
Depresión
/
Hipocampo
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS