Preliminary study of PRL-3 gene promoter binding sites of Snail in SW480 cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To identify the region in PRL-3 gene promoter where the transcriptional factor Snail can bind.</p><p><b>METHODS</b>PRL-3 promoter and the possible binding sites of the transcription factors were analyzed by bioinformatical methods. Chromatin immunoprecipitation and PCR were performed using the antibody specific for Snail to verify the binding of Snail to PRL-3 promoter.</p><p><b>RESULTS</b>According to the prediction by TRED, a promoter prediction software, PRL-3 gene promoter was located between -700 bp to 299 bp of PRL-3 gene. Many possible transcription factor binding sites such as for Snail, n-MYC, ARNT, E74A, NF-kappaB, NRF-2 and AML-1 were predicted by Consite, a promoter analysis web system. Interestingly, a 5'-CACCTG-3' core sequence and other related sequences of Snail binding sites were found in the promoter region of PRL-3 genes by Consite software. Two regions in PRL-3 promoter were validated to allow binding of Snail by chromatin immunoprecipitation analysis of SW480 cells.</p><p><b>CONCLUSIONS</b>Snail regulates the activity of PRL-3 gene by binding to the promoter of PRL-3 gene in SW480 cells.</p>