Effect of platelet CD42a modification by mPEG-SPA with different molecular masses / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect platelet antigen modification by mPEG-SPA with different molecular masses.</p><p><b>METHODS</b>Platelet CD42a was modified by 5 kD and 20 kD mPEG-SPA, respectively, and the fluorescence intensity of CD42a was detect by flow cytometry and the three-dimensional structure of CD42a simulated to analyze the distribution of lysine in CD42a molecule.</p><p><b>RESULTS</b>After platelet CD42a modification by 5 kD and 20 kD mPEG-SPA, the fluorescence intensity of CD42a decreased sharply by 85.54% and 88.65%, respectively, and multiple lysine regions were identified on the surface of CD42a molecule.</p><p><b>CONCLUSION</b>Both 5 kD and 20 kD mPEG-SPA allow useful modification of platelet CD42a, but 20 kD mPEG-SPA is more advantageous than 5 kD mPEG-SPA.</p>