Process of HIV-1 reverse transcription and its detection by using PCR / 药学学报
Acta Pharmaceutica Sinica
;
(12): 118-122, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-268160
ABSTRACT
Human immunodeficiency virus (HIV) is a retrovirus, belongs to Lentiviridae family. As long as viral genetic material entering into host cytoplasm, double-strand DNAs synthesis occurs which is catalyzed by reverse transcriptase (RT) with viral plus-strand RNA as template. This reverse transcription is a key link of HIV-1 life cycle and an important target for anti-HIV drug development. The process of reverse transcription can be divided into several steps:
formation of minus-strand strong-stop DNA; the first translocation; initiation of plus-strand DNA synthesis; and, the second translocation and the completion of both strands. These steps can be detected individually by using polymerase chain reaction (PCR) according to the amplified products on the region of R/U5, U3, U5/PBS and the sequence between LTR and Gag. In this review, we summarize the principle for detecting stages of HIV-1 reverse transcription by using PCR.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN Viral
/
ARN Viral
/
Reacción en Cadena de la Polimerasa
/
VIH-1
/
Transcripción Reversa
/
Replicación del ADN
/
Transcriptasa Inversa del VIH
/
Genética
/
Metabolismo
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2008
Tipo del documento:
Artículo
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