Study on neuronal apoptosis induced by hypoxia or traumatic injury in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 149-152, 2000.
Artículo
en Inglés
| WPRIM
| ID: wpr-268519
ABSTRACT
OBJECTIVE:
To explore the relationship between neuronal apoptosis and hypoxia or traumatic injury.METHODS:
Rat neurons primarily cultured in vitro were treated w ith hypoxia (the hypoxia group) or traumatic injury (the trauma group). The neur onal apoptosis was evaluated with microscope, TUNEL (terminal deoxynucleotidyl t ransferase mediated X-dUTPnick end labeling) staining, flow cytometry, agarose gel electrophoresis and immunohistochemistryRESULTS:
Morphological changes of apoptosis appeared in the t reated neurons and the DNA fragmentation showed "ladder" break. The apoptotic index was 10.8% in the hypoxia group and 4.8% in the trauma group, while it was only 1.6% in the control group. The expression of apoptosis-associated genes (c-myc, fas and fasL) increased.CONCLUSIONS:
Hypoxia or traumatic injury can induce neuronal ap optosis, and its molecular mechanism is probably related to the expressions of a poptosis-associated genes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Inglés
Revista:
Chinese Journal of Traumatology
Año:
2000
Tipo del documento:
Artículo
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