The cellular and molecular mechanism of inhibitory effect of asiaticoside on capsular contracture following breast augmentation / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 44-47, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-268647
ABSTRACT
<p><b>OBJECTIVE</b>To explore the cellular and molecular mechanism of the inhibitory effect of asiaticoside on capsular contracture following breast augmentation.</p><p><b>METHODS</b>Contracture capsule derived fibroblasts were cultured in medium with different concentration of asiaticoside. The cell proliferation, collage synthesis and alpha-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test.</p><p><b>RESULTS</b>DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asiaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1% respectively, showing a significant difference from that in control group (P < 0.05). The inhibitory effect increased with the rise of the asiaticoside concentration in a dose-dependent manner. When the concentration of asiaticoside reached 25 mg/L, the expression of alpha-SMA was down-regulated with an activation index of 1.673, showing a significant difference when compared with that in control group (P < 0.05).</p><p><b>CONCLUSIONS</b>asiaticoside can effectively inhibit the DNA and collagen synthesis of capsule-derived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Complicaciones Posoperatorias
/
Cirugía General
/
Triterpenos
/
Mama
/
ADN
/
Células Cultivadas
/
Colágeno
/
Mamoplastia
/
Contractura
/
Biología Celular
Límite:
Adulto
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Plastic Surgery
Año:
2011
Tipo del documento:
Artículo
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